2 . oligonucleotides based on sequence motifs ggvgktt and glplal 1 ( distance between them is about soobp ) conserved in tabacoo n and arabidosis i rps2 were used as pcr primers for scanning genomic dna . the amplified fragments of approximately 500bp were obtained in two different primer combinations , but were not considered as ht gene ' s analog for they also appeared in the genomic dna without ht gene 依据拟南芥rps2和烟草n基因中的两个保守序列ggvgktt和glplal (两者相距约500bp )合成寡核苷酸引物(左引物2个,右引物2个,组成4个引物组合) ,对玉米基因组dna进行扩增的结果表明:在2个引物组合中获得了大约500bp的扩增产物,但这两个扩增产物同样也存在与不含ht基因的玉米基因组中,说明这两个500bp左右的dna片断并非ht基因的类似序列。
Main methods and results are as followed : 1 epitope analysis of agonist - binding region of nrla physicochemical properties and antigenicity of two agonist - binding regions of nrla were analyzed through bioinformatics : domain p1 containing 151 amino acid residues preceding the first transmembrane domain of the human nrla , domain p2 with 144 residues following the third transmembrane domain . four parameters including hopp - woods and kyte hydrophilicityjanin accessibility , karplus - schulz flexibility , and welling antigenicity were used to determine the antigenic sites , and prosite programme and chou - fasman method were employed to analyze their related sequence motif and the secondary structures 用goldkey软件分别选取公认的hopp等与kyte等亲水性参数、 jain表面可及性参数、 karplus - schulz主链柔韧性参数及welling抗原性参数对p1 、 p2两个多肽片段进行参数分析。并采用通用的prosite程序与chou - fasman方法比较分析p1 、 p2多肽片段的氨基酸位点与二级结构特征。综合判定两个多肽片段的抗原性及其位点,结果认为p2抗原性强于p1 。
This article mainly discusses the challenges , research approaches and recent developed tools in the field of protein function prediction and the ways by which these issues change the process of drug discovery , including homology - based annotation transfer , sequence motifs and patterns , information on 3d structure , sub - cellular localization , posttranslational modifications , binding sites and functional residues , protein - protein interactions 摘要简述了在蛋白质功能预测领域中的研究方法和最新研发工具所面临的挑战,并讨论蛋白质功能预测是如何改变药物开发进展的,具体包括:基于序列同源性分析的注释转移、序列基元和模式增加了注释转移的说服力、 3d结构信息可以精炼注释转移、亚细胞定位、翻译后修饰、结合位点和功能残基、蛋白质之间的相互作用。
In genetics, a sequence motif is a nucleotide or amino-acid sequence pattern that is widespread and has, or is conjectured to have, a biological significance. For proteins, a sequence motif is distinguished from a structural motif, a motif formed by the three dimensional arrangement of amino acids, which may not be adjacent.